
die Wirtin
innkeeper
A woman who runs or owns a pub, inn, or restaurant.
Die Wirtin hat uns persönlich einen Tisch zugewiesen.
The innkeeper personally assigned us a table.
innkeeper / landlady
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innkeeper
A woman who runs or owns a pub, inn, or restaurant.
Die Wirtin hat uns persönlich einen Tisch zugewiesen.
The innkeeper personally assigned us a table.

landlady
A woman who rents out rooms, an apartment, or a house to others; a female landlord.
Meine Wirtin hat angekündigt, dass die Miete im nächsten Jahr erhöht wird.
My landlady announced that the rent will be increased next year.
'Die Wirtin' is the feminine form of 'der Wirt' (innkeeper/landlord). The ending '-in' is used as standard in German to form the female version of many professions or terms for people.
Die neue Wirtin des Lokals ist sehr freundlich.
The new innkeeper of the restaurant is very friendly.
You can tell from context whether it means the woman who runs a restaurant or a landlady. Words like 'Restaurant' (restaurant), 'bestellen' (to order), or 'Kneipe' (pub/bar) point to hospitality, while 'Miete' (rent), 'Wohnung' (apartment), or 'Vertrag' (contract) point to a landlady.
Die Wirtin brachte uns die Speisekarte.
The innkeeper brought us the menu.
In the context of a pub or restaurant, typical actions are 'bei der Wirtin bestellen' (to order from the innkeeper), 'die Wirtin nach der Rechnung fragen' (to ask the innkeeper for the bill), or 'von der Wirtin begrüßt werden' (to be greeted by the innkeeper).
Wir haben bei der Wirtin noch eine Runde Getränke bestellt.
We ordered another round of drinks from the innkeeper.
When it is about a landlady, typical phrases are 'die Miete an die Wirtin zahlen' (to pay the rent to the landlady), 'sich bei der Wirtin beschweren' (to complain to the landlady), or 'einen Termin mit der Wirtin haben' (to have an appointment with the landlady).
Bei Problemen mit der Heizung sollen wir uns direkt an die Wirtin wenden.
If there are problems with the heating, we should contact the landlady directly.
hostess / host
Wirtin and Gastgeberin can both refer to a woman who receives and looks after guests. Wirtin, however, emphasizes her role as the manager or owner of a restaurant or other establishment, while Gastgeberin emphasizes the social role of welcoming guests. Use Wirtin for the venue and Gastgeberin for the occasion or the home.

Die Wirtin begrüßte die Gäste und führte sie zu ihren Plätzen.
The innkeeper welcomed the guests and showed them to their seats.
Die Gastgeberin begrüßte die Gäste und bot ihnen Getränke an.
The hostess welcomed the guests and offered them drinks.