die Wirtin
innkeeper
A woman who runs or owns a pub, inn, or restaurant.
Die Wirtin hat uns persönlich einen Tisch zugewiesen.
The innkeeper personally assigned us a table.

innkeeper / landlady
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innkeeper
A woman who runs or owns a pub, inn, or restaurant.
Die Wirtin hat uns persönlich einen Tisch zugewiesen.
The innkeeper personally assigned us a table.

'Die Wirtin' is the feminine form of 'der Wirt' (innkeeper/landlord). The ending '-in' is used as standard in German to form the female version of many professions or terms for people.
Die neue Wirtin des Lokals ist sehr freundlich.
The new innkeeper of the restaurant is very friendly.
You can tell from context whether it means the woman who runs a restaurant or a landlady. Words like 'Restaurant' (restaurant), 'bestellen' (to order), or 'Kneipe' (pub/bar) point to hospitality, while 'Miete' (rent), 'Wohnung' (apartment), or 'Vertrag' (contract) point to a landlady.
Die Wirtin brachte uns die Speisekarte.
The innkeeper brought us the menu.
In the context of a pub or restaurant, typical actions are 'bei der Wirtin bestellen' (to order from the innkeeper), 'die Wirtin nach der Rechnung fragen' (to ask the innkeeper for the bill), or 'von der Wirtin begrüßt werden' (to be greeted by the innkeeper).
Wir haben bei der Wirtin noch eine Runde Getränke bestellt.
We ordered another round of drinks from the innkeeper.
When it is about a landlady, typical phrases are 'die Miete an die Wirtin zahlen' (to pay the rent to the landlady), 'sich bei der Wirtin beschweren' (to complain to the landlady), or 'einen Termin mit der Wirtin haben' (to have an appointment with the landlady).
Bei Problemen mit der Heizung sollen wir uns direkt an die Wirtin wenden.
If there are problems with the heating, we should contact the landlady directly.
innkeeper / host
'der Wirt' and 'die Wirtin' both mean a person who runs or owns a pub, inn, or restaurant. The most important difference is gender: 'der Wirt' is male, 'die Wirtin' is female. The words can be confused because both describe the same role in a restaurant. So pay attention to whether you are talking about a man or a woman. 'die Wirtin' also has another meaning as a landlady, but this relation is about hospitality and restaurants.

Die Wirtin begrüßte die Gäste persönlich an der Tür.
The innkeeper personally greeted the guests at the door.
Der Wirt brachte uns nach dem Essen noch einen Kaffee.
After the meal, the innkeeper brought us another coffee.